TRITC (Tetramethylrhodamine isothiocyanate)
by TdB Labs
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CAS Number: 95197-95-8
TRITC (Tetramethylrhodamine isothiocyanate) is a prominent member of the family of rhodamine dyes. Its high brightness and fluorescence quantum yield along with its capacity to covalently conjugate to proteins and polysaccharides efficiently under relatively mild conditions, render this rhodamine dye an indispensable fluorescent labeling agent.
Structure
TRITC is currently available in the form of a mixture of two isomers (5- and 6- isothiocyanate isomers). Depending on the isomer, TRITC contains an isothiocyanate group either at position 5 (isomer I) or position 6 (isomer 2) of the bottom benzene ring. Isothiocyanate easily reacts with nucleophiles such as amines under mild conditions. The compound is often used in the form of one of its two isomers or sometimes as a mixture of the two.
Spectral data
It exhibits an excitation maximum at λ = 560 nm and emission maximum at approximately λ = 590 nm in DMSO solutions
Storage and stability
TRITC should be stored in a dry environment. When desiccated, it can be safely stored at room temperature. However, it is generally recommended to store it at temperatures between 4-8 °C.
Solubility
TRITC (isomer mix.) is highly soluble in polar organic solvents such as DMSO and DMF. It also exhibits significant solubility in alcohols such as methanol and ethanol.
Applications
TRITC can efficiently and covalently conjugate to -NH₂ containing biomolecules such as proteins and antibodies or biorelevant macromolecules such as modified polysaccharides (e.g. lysine-dextran) at mild basic conditions. TRITC and TRITC-labelled products (e.g. TRITC-dextran) are highly important for a range of biorelevant applications e.g. cell imaging, permeability and microcirculation studies, as well as research on drug delivery as a molecular size marker and in various biosensor applications.
Technical information
Specifications