by TdB Labs
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CAS Number: N/A
Alternative Name: FTD
FITC-TRITC-dextran (FTD) is a dextran derivative that is labelled with two fluorophores, FITC and TRITC and it is currently produced at a molecular weight of 500 kDa.
Structurally, each of the fluorophores (FITC and TRITC) are bound to dextran through thiourethane linkages. The degree of substitution (DS) lies in the range of 0.001 to 0.020 mol of dye per mole of glucose units for each of the fluorophores. The molar ratio of FITC/TRITC bound to dextran can vary between 1:1 and 2:1 and it is assessed spectrophotometrically at a neutral pH.
As a result of the dual fluorescent labelling of dextran, FITC-TRITC-dextran exhibits fluorescence emission at λfl=517±5nm when photoexcited at λex=493 nm (due to FITC) and at λfl=575±5nm when photoexcited at λex=550 nm (due to TRITC).
Storage and Stability
FTD500 should be stored in a well-sealed container in the dark, at room temperature.
FTD500 is delivered as a pink powder, that is highly soluble in water and DMSO. However, it is incompatible with strong oxidizing agents.
What renders this TdB Labs product unique is that the fluorescence (as well as the absorbance) of one of the fluorophores (FITC) is highly dependent on pH while the corresponding ones of the other fluorophore (TRITC) remains nearly unchanged between pH 2 and 10. This makes FTD500 highly suitable for applications that allow accurate determination and monitoring of pH in living cells or tissues at a wide range of pH spanning from 3.5 to 8.0. This product can thus serve as an invaluable tool for intravital microscopy.