FITC (Fluorescein isothiocyanate)
by TdB Labs
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CAS Number: 3326-32-7
FITC (isomer I) is a derivative of fluorescein. FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. FITC can be easily used as labelling agent of various substrates such as proteins or polysaccharides. Isothiocyanate easily react with nucleophiles such as amines under mild conditions. FITC can also be used in flow cytometry.
Fluorescein isothiocyanate (widely known as FITC) is a derivative of fluorescein which contains an isothiocyanate group at either position 5 or position 6 of the bottom benzene ring.
FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm.
Storage and stability
Store at 0-8 ˚C in a tightly closed container when not in use. FITC is stable under recommended storage conditions
FITC has a wide-range of applications. Including fluorescent labelling of antibodies and flow cytometry. FITC-functionalized products e.g. FITC-dextrans are very useful for studying permeability and transport in a large variety of cells and tissues e.g. intestinal, neoplastic, and ocular tissues as well in research related to brain and neural system.Technical information