Eosin-Y-lysine-dextran 500
by TdB Labs
Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.
CAS Number: N/A
Alternative Name: EYLD500
Eosin-Y-lysine-dextran 500 (EYLD500) is a dextran derivative that is labelled with the Eosin Y dye and it is currently produced at a molecular weight of 500kDa.
Structure
Eosin-Y-lysine-dextran 500 (EYLD500) is a dextran-based derivative labeled with the dye Eosin Y (2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl) benzoate; CAS No: 17372-87-1). Lysine-dextran is marked using a special technique created by TdB Labs, which enables a significant level of functionalization. The method utilizes the amino groups of lysine dextran to achieve the formation of a linker that involves amide groups. The latter provides substantial spacing between the substrate and the fluorophore, and at the same time offers H-bonding opportunities (intramolecular and/or intermolecular, e.g., with solvent molecules or biomolecules e.g. proteins).
Spectral data
Owing to the presence of Eosin Y, EYLD500 exhibits strong orange fluorescence (excitation max. at λ=530 nm and fluorescence max. at λ=548 nm in water). The fluorescence quantum yield for EYLD500 sample with a degree of fluorescent labelling of 0.002 mmol EY/g sample has been determined to be Φ=0.91.
Storage and Stability
EYLD500 should be stored in a well-sealed container in the dark, at room temperature, and has a shelf-life of 6 years under these conditions.
Solubility
EYLD500 is delivered as a pink amorphous powder readily soluble at concentrations close to 100 mg/ml in water and 50 mg/ml in DMSO, where mild heating (up to max 60oC) might be required. However, it is insoluble in methanol and ethanol.
Application
EYLD500 is part of a family of fixable fluorescent labeled lysine-dextran derivatives that has a range of potential applications in cell permeability studies, tissue and cell imaging, as a fluorescent nanocarrier for drug delivery applications, and as a pH indicator due to the pH-dependent fluorescence of the fluorophore (Eosin Y).
Technical information
Specifications